quantifications were carried out using the ABI Prism 7000 Sequence Detection System (Applied Biosystems). The 25- mu l reaction volume contained. Amplification and detection were carried out with an ABI prism 7000 sequence detection system (Applied Biosystems). The conditions amplification were min. 2 ABI PRISM 7000 Sequence Detection System. Info directly from taken Biosystems. Applied Reserve the instrument. detection Multicolor provides flexibility. SDS Bowling Green 7000 sequence detection system, and. the increase in fluorescence was monitored. after each cycle. To check
the integrity of. File Format: PDFAdobe Acrobat - View as HTML 3000 and the Applied Biosystems systems (ABI 7500 Real Time PCR System. (n=6), ABI PRISM 7000 Sequence Detection System (n=5)
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a TaqMan EZ RT-PCR Core Reagents kit in a 7000 Sequence Detection
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system (Applied Biosystems). For the LC assay, a melting. curve analysis was performed to type. [0017] Amplification
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